Abstract: A self-designed automatic analytical system with in-line extraction and separation of Cr(Ⅵ) in combination with inspection by ICP-AES was applied to the determination of Cr(Ⅵ) in gelatin capsules. 2.0 g of the sample was extracted ultrasonically in an atmosphere of argon for 15 min with 100 mL of PBS of pH 8.0 and 0.4 g of anhy. MgCl2. The acidity of the extract was adjusted to pH 5.0, after filtration and de-coloration, and Cr(Ⅵ) in the solution was adsorbed on the ion exchange column, and eluted from the column with 0.4 mol·L-1 HNO3 solution (containing 1 g·L-1 of ascorbic acid). The eluate was introduced into the detection unit of the analytical system. Linear relationship between values of emission intensity and mass concentration of Cr(Ⅵ) was kept in the range within 100 μg·L-1, with detection limit (3s) of 0.30 μg·L-1. Values of RSD′s (n=6) found were ranged from 1.9% to 3.6%. Value of average recovery found by standard addition method was 99.1%.