Abstract: A method of HPLC for the determination of 9 active ingredients in Arundina Graminifolia was proposed. The sample was extracted with methanol (8+2) solution by speedy and homogeneous pulping, and an aliquot of 5.0 mL of the extract was purified by passing through MCI-GEL RP-resin SPE column. An aliquot of 2.00 mL of the eluate was taken for HPLC analysis. Waters Xbridge chromatographic column was used as stationary phase and mixtures of methanol and acetic acid (0.5+99.5) solution with different mixing ratios were used as mobile phase in the gradient elution. UV-detection at 275 nm was adopted in the determination. Linear relationships between values of peak area and mass concentration of the 9 active ingredients were kept in definite ranges. Detection limits (3S/N) found were ranged from 20 to 30 μg·L-1.Values of recovery found by standard addition method were in the range of 96.4%-105%, with values of RSD′s (n=7) in the range of 1.5%-2.4%.