Abstract: A method of HPLC combined with molecular imprinting matrix solid phase dispersion was applied to determine amlodipine besylate in blood.Molecularly imprinted polymer for amlodipine besylate was synthesized and mixed with the sample, used as matrix solid phase dispersing agent. After air-drying, the mixture was used as filter for packing column, and amlodipine besylate was eluted from the column by methanol.The eluent was separated on an Inertsil DOS-SP column using a mixture of methanol-acetonitrile-water (70+22+8) as mobile phase. UV absorbance was measured at 237 nm. The linearity range of amlodipine besylate was 1.7-20.0 μg·L-1, with detection limit (3S/N) of 0.5 μg·L-1 and lower limit of determination (10S/N) of 1.7 μg·L-1. The recovery rates measured by standard addition method were in the range of 91.0%-109%, with RSD (n=5) of 3.7%-4.8% . The method was used for measurement of amlodipine besylate in blood.