Abstract: Samples of textile were treated by the accelerated solvent extraction (ASE) and sweat simulant extraction (SSE), to separate 11 carcinogenic dyestuffs and their contents in the extract were determined by UHPLC-MS/MS. For separation of the dyestuffs, Hypersil Gold chromatographic column was used as stationary phase, and mixtures of tetrabutyl ammonium dihydrogen phosphate solution of pH 7.5 and acetonitrile in different ratios were used as mobile phase in the gradient elution. The ionization modes of ESI+ and ESI- with MRM mode of detection were adopted in MS/MS determination, with quantification by external standard method. Linearity ranges of the 11 dyestuffs were kept same from 1.0 to 1 000 μg·L-1 with detection limits (3S/N) in the range of 0.5-30.0 μg·L-1. Values of recovery of the 11 dyestuffs were in the range of 9.12%-102.4% obtained by ASE and in the range of 0.72%-2.18% obtained by SSE.