Abstract: Capillary electrophoreto-condustometry was applied to the determination of hypocrellin A in Shiraia bambusicola P. Henn, using acetate buffer of 20 mmol·L-1 NH4OAc solution (pH 3.5, adjusted with 0.1 mol·L-1 HOAc solution) as electrophoretic medium, and 20 kV as separation voltage. Ethanol was added to the buffer solution amounting to 5% by volume to improve solubility of the sample and to control the magnitude and direction of electroosmotic flow. Sample was introduced by siphoning at a height of 20 cm for 10 s. Column-end detection of conductance was adopted in the measurements. Range of linearity was found between 1.0 and 160.0 mg·L-1 of hypocrellin A, with detection limit (3S/N) of 0.5 mg·L-1. Results of recovery test found were ranged from 99.0% to 100.2%.