Abstract: HPLC was applied to the determination of 4 artificial edulcorators, i.e., acesulfame, sodium saccharin, aspartame and neotame in milk and its products. The sample was added with KFe(CN)3 and Zn(OAc)2 to precipitate protein, and the edulcorator was separated by gradient elution with a mixed solution of 0.05 mol·L-1 KH2PO4 and acetonitrile in different ratios on Diamonsil-C18 column (250 mm×4.6 mm, 5 μm). Diode array detector was used for the determination of acesulfame at 230 nm and for sodium saccharin, aspartame and neotame at 210 nm. Values of detection limits (3S/N) of the method for the 4 edulcorators were found to be 1.0, 0.5, 1.5 and 2.5 μg·g-1 respectively. Using blank sample of milk powder as matrix, recovery test was made by standard addition method, values of recovery were found in the range of 90.0%-103.3% and RSD′s (n=8) found were ranged from 2.1% to 5.8%.