Abstract: AuNPs with good biocompatibility were dropped onto the surface of a glassy carbon electrode, then human enterovirus 71 (EV71) immunosensor was formed by immobilization of anti-EV71 on AuNPs. The electrochemical immunosensor could be used for sensitive detection of EV71.The performances of the as-prepared immunosensor were investigated by cyclic voltammetry in PBS (pH 7.0) containing 0.1 mol·L-1 KCl and 5 mmol·L-1 K3Fe(CN)6/K4Fe(CN)6. The oxidation peak current of redox probe, ［Fe(CN6)］3-/4-, decreased linearly over the EV71 mass concentration ranged from 0.1 to 80 μg·L-1 with detection limit (3S/N) of 0.025 μg·L-1. Precision of the method was tested at the concentration level of 20 μg·L-1 anti-EV71 standard solution for 5 determinations, value of RSD found was 3.0%. On the base of blank sample, test for recovery was made by standard addition method; values of recovery found were in the range of 98.3%-100%.