Abstract: The chemiluminescence (CL) produced by the reaction between luminol and N-bromosuccinimide (BSMD) in Na2CO3-NaHCO3 buffer medium was found to be strongly enhanced by the presence of halcinonede,an adrenocorticomimetic drug,and the magnitude of increase in CL-intensity was found to keep linear relationship with the concentration of halcinonede in the range of 1.0×10-6-1.0×10-4g·L-1,with a detection limit (3S/N) of 3×10-7g·L-1.Based on these facts and by applying the flow injection technique,a method of FI-CL determination of halcinonede in its preparation was proposed.Precision of the method was tested at the concentration level of 4.0×10-5g·L-1 by 11 determinations,and the value of RSD obtained was 3.0%.In analyzing 3 substantial samples of the drug,results obtained by this method were in consistency with the results found by the method of pharmacopoeia.