流动注射-化学发光法测定片剂中左炔诺孕酮含量

FI-Chemiluminescence Determination of Levonorgestrol in Its Tablets

摘要: 在酸性条件下,高锰酸钾与亚硫酸钠能够产生较弱的化学发光,而左炔诺孕酮能够增强该化学发光强度.在一定浓度范围内,增加的发光强度与左炔诺孕酮的浓度呈线性关系,由此建立了一种测定左炔诺孕酮的流动注射化学发光方法.方法的检出限(S/N=3)为6.4 μg·L-1,线性范围为0.01~0.5 mg·L-1和0.5~5.0 mg·L-1,对0.1 mg·L-1左炔诺孕酮平行测定11次,其相对标准偏差为4.3%.

Abstract: In a dil.H2SO4 medium,weak chemiluminescence (CL) was produced by the reaction between KMnO4 and Na2SO3,and was enhanced to a great extent by the presence of levonorgestrol (abbreviated as LNGT).It was found that in the concentration ranges of 0.01 to 0.5 mg·L-1 and 0.5 to 5.0 mg·L-1,linear relationships between the magnitude of increase in CL intensity and concentration of LNGT were obtained.Based on these facts,a new FI-CL method for determination of LNGT in its tablets was proposed with a detection limit (S/N=3) of 6.4 μg·L-1.Precision of the method was tested at the concentration level of 0.1 mg·L-1 of LNGT with 11 determinations,giving value of RSD of 4.3%.