Abstract: A method of HPLC for determination of trace amount of formaldehyde in wine with derivatization by Hantzsch reaction was proposed. The optimized conditions for derivatization reaction found were as follows: ① acidity of the reaction system: pH 5.5; ② temperature of reaction: 50 ℃; ③ time of reaction: 20 min; ④ amount of acetylacetone (0.2+100) solution added: 0.5 mL; ⑤ concentration of ammonium chloride solution acidity: 0.5 mol·L-1. Linear relationship between values of peak area and mass concentration of formaldehyde was kept in the range of 2-2 000 μg·L-1, with detection limit (3S/N) of 0.2 μg· L-1.The proposed method was applied to the analysis of samples of beer and white spirit, giving values of recovery by standard addition method in the range of 81.2%-102%.