高效液相色谱法快速测定三白草中黄酮类物质

Rapid Determination of Flavonoid in Saururus Chinesis (Lour) Baill by High Performance Liquid Chromatography

摘要: 研究了用固相萃取预分离,高效液相色谱法测定三白草中黄酮类物质.三白草中的黄酮用甲醇(90+10)加热回流提取,提取液用Waters Sep-Pak-C18固相萃取小柱预分离,以ZORBAX Stable Bound (4.6 mm×50 mm,1.8 μm)色谱柱为固定相,磷酸(2+998)和甲醇(以体积比50比50)为流动相,在该色谱条件下,三白草中主要的黄酮成分在1.5 min内可达到基线分离;用紫外二极管矩阵检测器检测.用该方法测定了5种三白草样品中的黄酮类物质,回收率在97%~103%之间,相对标准偏差在1.6%~2.2%之间.

Abstract: The sample was pretreated by refluxing with CH3OH+H2O(90+10) for 45 min and extracting on Sep-Pak-C18 microcolumn.In the HPLC analysis,the Zorbax stable Bound (4.6 mm×50 mm,1.8 μm) was used as stationary phase,and a mixture of H3PO4 (2+998) and methanol with a ratio of 1 to 1 was used as the mobile phase.Main flavones in the sample were separated from the base-line within 1.5 min.UV-diode array detector was used in the determination at the wavelength of 360 nm.Samples of 5 kinds of saururus chinesis (Lour) baill was analyzed by the proposed method,giving values of recovery in the range 97%-103%,and RSD′s (n=5) in the range 1.6%-2.2%.