流动注射化学发光法测定茶叶和蔬菜中亚硝酸根

FI-Chemiluminescence Determination of Nitrite in Tea and Vegetables

摘要: 利用亚硝酸根对鲁米诺-溴酸钾体系的化学发光具有显著增强作用,结合流动注射技术,建立了一种简单、快速测定亚硝酸根方法.方法的线性范围为4.0×10-3~4.0 mg·L-1;检出限为2.0×10-3mg·L-1(3σ);分别对 0.01 mg·L-1和0.40 mg·L-1的亚硝酸根进行了平行测定(n=11),其相对标准偏差分别为1.3%与1.5%.应用于茶叶、蔬菜中的亚硝酸根的测定,回收率为92.3%~108.0%.

Abstract: Based on the enhancement effect of NO-2 on the chemiluminescence (CL) reaction system of luminol and potassium bromate,and applying the flow injection (FI) technique,a new FI-CL method for determination of NO-2 was proposed.Linearity was kept in the range of 4.0×10-3-4.0 mg·L-1 of NO-2,with a detection limit (3σ) of 2×10-3mg·L-1.Precision was tested at the NO-2 concentration levels of 0.01 mg·L-1 and 0.40 mg·L-1,the values of RSD′s (n=11) obtained were 1.3% and 1.5% respectively.In the application of the proposed method to the determination of NO-2 in tea and vegetable samples,recoveries in the range of 92.3%-108.0% were obtained.