Abstract: A method for the determination of 8-hydroxy-2′-deoxguanosine (8-OHdG) in urine sample by CE based on enrichment by online sweeping with nanogold was proposed. Analytical conditions were studied and following optimized parameters were given: ① moving voltage: -20 kV; ② electrophoretic medium: 20 mmol·L-1 of borax solution (pH 8.2, including 10 nm nanogold solution of 200 μL and 0.1 mmol·L-1 CTMAB; ③ wavelength of detection: 254 nm. It was shown that micelle was formed between nanogold and CTMAB in the electrophoretic medium, which conduced to the sweeping of 8-OHdG. The baseline resolution was attained within 10 min. Linearity range between 0.50 and 50.0 μmol·L-1 was obtained with detection limit (3S/N) of 39 nmol·L-1. The proposed method was used in the determination of 8-OHdG in a urine samples, gving recovery found by standard addition method in the range of 90.0%-104.6%.