高效液相色谱法测定葛粉保健品中葛根素的含量
HPLC Determination of Puerarin in An Health-care food “Kudzu Vine Root Powder”
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摘要: 对不同产区葛粉保健品中葛根素的含量进行分析,拟建立高效液相色谱法快速测定葛粉保健品中葛根素含量的方法.固定相为Agilent-C18柱(4.6 mm×300 mm,5 μm),流动相为甲醇-水(70+30,体积比)溶液,流速为1.0 mL·min-1,波长为250 nm,进样量10 μL.结果表明:葛根素的质量浓度在10.00~100.00 mg·L-1范围内与峰面积呈线性关系,加标回收率为99.0%~99.8%,检出限(3S/N)为1.0 mg·L-1,相对标准偏差分别为0.57%,0.64%,1.71%.Abstract: Concents of puerarin in kudzu vine root powder produced in different areas in our country were determined by HPLC.The Agilent-C18 column (4.6 mm×300 mm,5 μm) was used as the stationary phase,and a mixfure of methanol and H2O,mixed in the volume ratio of 70 to 30,was used as the mobile phase with a flow-rate of 1.0 mL·min-1.Volume taken for sample introduction was 10 μL.UV-detections were made at the wavelength of 250 nm.Linear relationship was obtained in the range of 10.00 to 100.00 mg·L-1 between the values of peak area and concentration of puerarin,with its detection limit (3S/N) of 1.0 mg·L-1.Test for precision was made at 3 different concentration levels of 1.58,1.55 and 0.24 mg·L-1 for 6 determinations each,giving values of RSD′s of 0.57%,0.64% and 1.71% respectively.Under the condition of sample analysis,recovery of the method was tested by addition of standard solution of puerarin at 3 different concentrations,and values of recovery (n=6) obtained were in the range of 99.0% to 99.8%
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