Abstract: It was found that the chemiluminescence (CL) reaction between luminol and potassium ferricyanide was significantly sensitized by enoxacin,and that linear relationship between the enhanced CL signal (ΔI) and the concentration of enoxacin was held in the range of 2.0×10-8-2.0×10-5mol·L-1 with a detection limit (3S) of 5.7×10-9mol·L-1. Precision of the method was tested at the concentration level of 1.0×10-6mol·L-1 for 11 determination,giving value of RSD 1.75%. By applying the flow injection (FI) technique to the CL reaction system,a new FI-CL method was proposed for the determination of enoxacin in enoxacin tablets. In analyzing 3 samples of the drug tablets,results obtained by this method were in conformity with the labelled values. Test for recovery was made by standard addition method and results found were in the range of 96.7%-103.0%.