Abstract: A review on the significance of speciation analysis of selenium in plants,the species of selenium in plants,and the application of various analytical methods in hyphenation to the analysis of this objective was presented,covering mainly in the years from 1998 to 2009.Solvent extraction,liquid chromatography,gas chromatography,electrophoresis,and capillary electrophoresis were used as methods of separation and hyphenated individually with the detection methods of ICP-MS,isotope dilution-MS,electrospray MS or ICP-AES etc.to attain the speciation analysis of selenium in plants (51 ref.cited).