反相高效液相色谱法测定土壤中氰溴甲苯
RP-HPLC Determination of Bromo-cyano-methybenzene in Soil
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摘要: 提出了反相高效液相色谱法同时测定土壤中氰溴甲苯的方法.样品采用索氏提取法,以二氯甲烷为提取溶剂提取6 h.提取液以ODS-C18色谱柱(150 mm×6 mm,5 μm)为分离柱,以甲醇-水(70+30)为流动相经色谱分离,在检测波长为237 nm处进行测定.氰溴甲苯的质量浓度在0.6~10.0 mg·L-1范围内与其峰面积呈线性关系.标准加入回收率在85.0%~95.7%之间,相对标准偏差(n=6)在1.9%~10.3%之间.Abstract: RP-HPLC was applied to the determination of bromo-cyano-methybenzene in soil.The sample was treated by Soxhlet extraction with dichloromethane as extraction solvent for 6 h.The extract obtained was separated on ODS-C18 column (150 mm×6mm,5 μm) with a mixture of methanol-water (70+30) as mobile phase and the content of bromo-cyano-methybenzene was determined at the wavelength of 237 nm.Linear relationship between the values of peak area and mass concentration of bromo-cyano-methybenzene was obtained in the range of 0.6-10.0 mg·L-1.The values of recovery found by standard addition method were in the range of 85.0%-95.7%,and values of RSD′s (n=6) were in the range of 1.9%-10.3%.
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