离子色谱法测定水果中亚硝酸盐和硝酸盐

Ion Chromatographic Determination of Nitrite and Nitrate in Fruits

摘要: 建立了离子色谱法测定水果中亚硝酸盐和硝酸盐的方法.试样溶液中亚硝酸盐和硝酸盐在YSA-8阴离子色谱柱分离,以4 mmol·L-1碳酸钠-3 mmol·L-1碳酸氢钠为流动相,电导检测.亚硝酸盐和硝酸盐的检出限分别为0.02 mg·L-1和0.11 mg·L-1,工作曲线的线性范围分别在0.2~20.0 mg·L-1和0.3~30.0 mg·L-1范围内.该方法已应用于水果中亚硝酸盐和硝酸盐的测定,测得回收率在93.4%~105.2%之间.

Abstract: An ion chromatographic method for the determination of nitrite and nitrateions in fruits was proposed.Nitrite and Nitrate in sample solution were separated on an anion separation column YSA-8 and eluted with a mobile phase consisted of 4 mmol·L-1 Na2CO3 and 3 mmol·L-1 NaHCO3.The eluate was detected with electroconductivity detector.DL of the method was 0.02 mg·L-1 for Nitrite and 0.11 mg·L-1 for Nitrate,respectively.Linearity of the calibration curve was ranged in 0.2-20.0 mg·L-1 for Nitrite and in 0.3-30.0 mg·L-1 for Nitrate,respectively.The proposed method was applied to the determination of Nitrite and Nitrate in fruit samples giving values of recovery in the range of 93.4%-105.2%.