基于铅(Ⅱ)-二甲酚橙配合物反应光度法测定血清蛋白

Photometric Determination of Serum Albumen Based on Its Reaction with the Coordination Complex of Lead(Ⅱ) and Xylenol Orange

摘要: 在pH 6.1的磷酸氢二钠-柠檬酸钠-磷酸缓冲溶液中,血清蛋白与铅-二甲酚橙反应生成复合物,最大吸收波长为300 nm,以牛血清蛋白为分析物,当牛血清蛋白的质量浓度在7 mg·L-1以内遵守比耳定律,其检出限(3S/N)为7.4×10-3mg·L-1.应用所提出的方法分析了3组人血清蛋白试样,所得结果用常规的CBBG-250方法作了校核.

Abstract: In a buffer solution of disodium hydrogenphosphate,sodium citrate and phosphoric acid of pH 6.1,a compound complex was formed between serum alumumen and the complex of Pb2+ with xylenol orange (XO),having its absorption maximum at 300 nm.Taking bovine serum albumien (BSA) as analyte,beer′s law was obeyed within 7 mg·L-1,with a detection limit of 7.4×10-3mg·L-1.The proposed method was used in analysis of 3 human serum albumen samples,and the results obtained were checked by the conventional method of CBBG-250.