高效液相色谱法测定固体酸催化1,4-二甲氧基苯乙酰化反应产物

HPLC Determination Reaction Product of Solid Acid-Catalytic Acetylation of 1,4-Dimethoxybenzene

摘要: 提出了高效液相色谱法测定固体酸催化1,4-二甲氧基苯乙酰化反应产物2,5-二甲氧基苯乙酮的方法.高效液相色谱柱为ODS-C18反相柱(250 mm×4.6 mm,5 μm),甲醇-水(80+20)溶液为流动相,检测波长为250 nm.在此检测条件下,2,5-二甲氧基苯乙酮检测量在 0.277~2.777 mmol·L-1范围与其峰面积呈线性关系.该方法可迅速准确地用于固体酸催化的1,4-二甲氧基苯乙酰化反应转化率的测定.

Abstract: HPLC was applied to determination of reaction product,namely 2,5-dimethoxyacetophenone,of the solid acid catalytic acetylation of 1,4-dimethoxybenzene.ODS-C18 RP column (250 mm×4.6 mm,5 μm) was used as stationary phase,and ethanol-H2O (80+20) solution was used as mobile phase.UV-detection at the wavelength of 250 nm was adopted in the measurement.Under the prescribed conditions,linearity range of 0.277-2.777 mmol·L-1 of 2,5-dimethoxyacetophenone was found with the values of peak areas.The proposed method was used to monitor and control the conversion rate of the acetylation of 1,4-dimethoxybenzene.