Abstract: GC was applied to the determination of 11 chlorobenzenes in aquaculture water with static headspace. The optimum experimental conditions found were to put 10 mL water sample containing 200 g·L-1 sodium chloride in a 20 mL vial and stir at 70 ℃ for 30 mins. 11 chlorobenzenes were separated completely by DB-35 MS capillary column (30 m×0.25 mm, 0.25 μm), detected by electron capture detector and then quantify through external standard method or standard addition method. The linearity of the method ranged from 0.16 to 8.0 μg·L-1 for dichlorobenzene isomers, 0.017 6-0.88 μg·L-1 for trichlorobenzene isomers, 0.004-0.2 μg·L-1 for tetrachlorobenzene isomers and from 0.001 to 0.05 μg·L-1 for pentachlorobenzene and hexachlorobenzene. The limits of detection ranged from 0.0002 to 0.04 μg·L-1. The proposed method was used in the analysis of aquaculture water samples, giving values of recovery and RSD′s (n=5)in the ranges of 86.0%-106% and 2.1%-5.8% respectively.