Abstract: The volatile oil in dalbergia odorifera was extracted by microwave assisted extraction of sample with cyclohexane as extractant.By using the orthogonal experimental designing,the technological conditions for extraction of volatile oil from dalbergia odorifera were determined as follows: ① m (Dalbergia odorifera)-V (cyclohexane) ratio: 20 to 250 (g∶mL);② time of microwave extraction: 10 min;③ power of extraction: 600 W;④ temperature of extraction: 60 ℃.The yield of volatile oil found under the optimum conditions was 1.98%.Tweenty one compounds were found and identified by GC-MS.The main compounds and their contents were found as follows: 2-amino- phenol (27.43%),nerolidol (24.83%),(E)-2-caren-4-ol (24.65%),2-(5-methyl-furan)-propionaldehyde (7.38%) and 1,1-dimethyl-2-(2-methyl-2-propenyl)-cyclopropane (4.67%).