高效液相色谱法测定发泡聚苯乙烯材料中六溴环十二烷含量

HPLC Determination of Hexabromocyclododecane in Expanded Polystyrene

摘要: 采用高效液相色谱法测定发泡聚苯乙烯材料中六溴环十二烷的含量.发泡聚苯乙烯材料样品用乙腈提取,以GL Sciences Inc Inertsil ODS-SP色谱柱(250 mm×4.6 mm,5 μm)为分离柱,以乙腈-水(85+15)溶液为流动相,在检测波长254 nm处进行测定.六溴环十二烷在一定的质量浓度范围内与其峰面积呈线性关系,方法的检出限(3S/N)在140~200 mg·kg-1之间.以空白样品为基体进行加标回收试验,所得回收率在88.6%~103%之间,测定值的相对标准偏差(n=7)在1.2%~6.5%之间.

Abstract: HPLC was applied to the determination of hexabromocyclododecane in expanded polystyrene.The sample of expanded polystyrene was extracted with acetonitrile.GL Sciences Inc Inertsil ODS-SP chromatographic column (250 mm×4.6 mm,5 μm ) was used as stationary phase,and a mixture of acetonitrile and water (85+15) as mobile phase.UV-detection at 254 nm was adopted in the determination.Linear relationship between values of peak area and mass concentration of the hexabromocyclododecane was kept in definite ranges,with detection limits (3S/N) in the range of 140-200 mg·kg-1.On the base of blank sample,test for recovery was made by standard addition method;values of recovery found were in the range of 88.6%-103%,with RSD′s (n=7) in the range of 1.2%-6.5%.