线性扫描伏安法测定人血清白蛋白

Linear Sweeping Voltammetric Determination of Human Serum Albumin and Its Analytical Application

摘要: 建立了一种测定人血清白蛋白的新方法.应用线性扫描伏安法研究了灿烂甲酚蓝(BCB)与人血清白蛋白(HSA)的相互作用的条件,当BCB与HSA作用后,其峰电流下降且峰电位负移,峰电流的下降值的二阶导数(ΔI″p)同HSA的质量浓度在2.0~60.0 mg·L-1范围内呈线性关系,相关系数为0.997 0.方法的检出限为2.0 mg·L-1，用于健康人血清样品的测定,回收率在95.6%~107.5%之间.

Abstract: Linear sweeping voltammetry was applied to the determination of human serum albumin (HSA) bese on its interaction with brillianl cresol blue (BCB).Conditions for the reaction were studied and optimized.In a B-R buffer medium of pH 6.5,reductive peak of BCB was observed at -0.1 V (vs.SCE),and in the presence of HSA,due to their interaction,the reductive peak was shifted negatively with decrease in peak current.Linear relationship between 2nd derivative of the magnitude of decrease in peak current (ΔI″p) and the concentration of HSA was found in the range of 2.0-60.0 mg·L-1 (r=0.997 0).The detection limit was found to be 2.0 mg·L-1.Test for recovery was made by standard addition method and the results obtained were in the range of 95.6%-107.5%.