Abstract: Three different methods were described for preparation of the coupled complex formed between sulfanilamide (SN) and water soluble CdTe quantum dots (QD′s),which were prepared by wrapping nanoparticles of the CdTe QD′s with mercaptopropionic acid (MPA).Structure and part of its spectroscopic features were characterized by UV-VIS spectrometry,fluorescence spectrometry,transmission electron microscopy and FT-IR spectrometry.As shown by the results,coordination was carried out through the reaction between the S of -SH group and the O of -COOH group in MPA and Cd2+ ions which were rich on the surface of the CdTe QD nanoparticles,and coupling reaction between the water soluble CdTe QD′s and SN was achieved through the intramolecular hydrogen bond formed between the O of -COOH group of MPA,surrounding the QD′s,and the -NH2 group of SN.And further,inhibitory effect of the coupled complex of CdTe QD′s-SN on proliferation of colon bacillus was also observed by biological test.As a conclusion,preparation of the coupled complex of CdTe QD′s with SN was proved to be surccessful by all the experimental results.