固相萃取-反相高效液相色谱法测定隐形眼镜护理液中聚胺丙基双胍

SPE-RHPLC Determination of Polyhexamethylenebiguanide in Lens Cleaning-Disinfectant Solution

摘要: 建立了固相萃取-反相高效液相色谱法测定隐形眼镜护理液中聚胺丙基双胍的方法.用Spe-ed WCX固相萃取柱(500 mg/3 mL)将样品浓缩富集后,以Shiseido C18色谱柱(150 mm×4.6 mm,5 μm)为分离柱,以pH 4.0的乙腈-20 mmol·L-1乙酸铵(体积比16比84)为流动相,检测波长为235 nm,7 min内测定样品中聚胺丙基双胍.方法检出限(S/N=3)为0.046 mg·L-1,相对标准偏差为3.3%(n=7),线性范围为100~800 mg·L-1,加标回收率93.5%~110.9%.

Abstract: SPE-RHPLC was applied to the determination of polyhexamethylenebiguanide (PHMBG) in lens cleaning-disinfectant solution.PHMBG was enriched on the Spe-ed WCX solid phase extraction column and separated on the shiseido C18 column.A mixed solution of acetonitrile and 20 mmol·L-1 ammonium acetate (mixed at a ratio of 16 to 84) at pH 4.0 was used as mobile phase.UV-detection at 235 nm was adopted in the determination.Linear relationship between values of peak area and concentration of PHMBG was obtained in the range of 100-800 mg·L-1,and detection limit (S/N=3) of the method was found to be 0.046 mg·L-1.Tests for recovery and precision were performed and results of recovery in the range from 93.5% to 110.9% and value of RSD′s (n=7) of 3.3% were obtained.Whole process of a determination was completed within 7 min.