Abstract: Contents of quercetin and kaempferol,as the active medicative components of Impatiens balsamina in different positions of its plant were determined by HPLC and a comparative study was made.The DiamonisilTM C18 (4.6 mm×250 mm,5 μm) column was used stationary phase,and a mixture of methanol and dil.acetic acid (0.5+99.5) mixed in the ratio of 65 to 35 (pH 3.5) was used a mobile phase with a flow-rate of 0.9 mL·min-1.UV-detection was made at the wavelength of 365 nm.Linear relationship between values of peak area and concentration was obtained in the range of 0.84-6.72 mg·L-1 for quercetin,and in the range of 2.56-20.48 mg·L-1 for kaempferol.Tests of recovery were made by standard addition method,and values of average recovery found were 101.1% and 96.0% with RSD′s (n=5) of 1.5% and 2.6% respectively.It was shown that highest content of quercetin (0.112 mg·g-1) was found in the leaves before blossoming and highest content of kaempferol (0.972 mg·g-1) was found in the flower.