Abstract: Wavelet transform method was applied to the IR-spectrometric determination of glucose in tomato to de-noise and smoothen the IR spectrum of tomato sample,resulting enhancement of resolution of the spectrum.In the determination,wave numbers close to 2 900 cm-1 (due to-CHO group) were chosen as the characteristic absorption peaks.By plotting values of mass (mg) of standard glucose (as independent variables m) against values of the respective absorbance (as dependent variables A),both the linear and non-linear mathematical models were established for the quantification.It was shown that the correlation coefficient of non-linear regression equation attained to a value of 0.938 3,while a value of only 0.801 5 was attained for the linear regression equation,and that results with higher accuracy were obtained by using the non-linear mathematical model,giving relative error of -2.29%.