Abstract: The sample of tobacco was combusted in oxygen bomb, and the SO2 evolved were absorbed and transformed to SO42- in 20 mL aqueous solution containing 0.25 mL of 30% hydrogen peroxide, and the excess hydrogen peroxide was removed by heating to boil for 10 min. The content of sulfur (as SO42-) in the absorption solution was determined by IC. Ionpac AS14 column was used as ion exchange column, and a mixture of 1.8 mmol·L-1Na2CO3 and 1.7 mmol·L-1 NaHCO3 was used as eluent. Linear relationship between values of peak area and mass concentration of sulfur was obtained in the range of 0.02-60 mg·L-1, with detection limit (3s) of the method of 3.24 μg·L-1. The proposed method was used in the analysis of standard sample of tea (GSB-7), the results obtained were in conformity with its certified values. Values of recovery found were in the range of 99.2%-99.7% with RSD′s (n=6) less tnan 1%.