离子色谱法测定银杏叶中无机阴离子
Ion-Chromatographic Determination of Inorganic Anions in Folia Gingko
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摘要: 应用离子色谱法测定了银杏叶中6种无机阴离子(F-,Cl-,NO-2,PO3-4,NO-3及SO2-4),测定中采用了Y5AB-8041A型阴离子分离柱及电导检测器,用含有5.0 mol·L-1碳酸钠溶液及5.4 mmol·L-1碳酸氢钠溶液的混合液作为洗脱液,以1.5 mL·min-1的流速通过分离柱.6种阴离子的峰高值与浓度值在一定范围内呈线性关系,其相关系数在0.999 1~0.999 7之间.测得6种阴离子的检出限(以μg·L-1表示)依次为2.75,4.71,9.43,11.1,30.0及22.4.用含6种阴离子的混合标准溶液,连续测定5次,检测方法的精密度,算得其相对标准偏差≤0.94%.用标准加入法测定了方法的回收率,测得结果在96.4%~102.3%之间.Abstract: Six inorganic anions (F-,Cl-,NO-2,PO3-4,NO-3 and SO2-4) in folia gingko were determined by ion-chromatography.An anion separation column of the model YSAB-8041A and conductivity detector were used in the determination.A mixed solution containing 5.0 mmol·L-1 Na2CO3 and 5.4 mmol·L-1 NaHCO3 solutions was used as eluant,passing through the column at a flow-rate of 1.5 mL·min-1.Linear relationships between values of peak height and concentration of the anions mentioned above were obtained with correlation coefficients ranging from 0.999 1 to 0.999 7.Detection limit (μg·L-1) were found as 2.75 (for F-),4.71 (for Cl-),9.43 (for NO-2),11.1 (for PO3-4),30.0 (for NO-3) and 22.4 (for SO2-4).Precision of the method was tested using mixed standard solution of the 6 anions with 5 successive determinations,giving values of RSD≤0.94%.Test for recovery was performed by the standard addition method,giving values of recovery in the range of 96.4%-102.3%.