Abstract: A method of GC-MS for the determination of volatile and semi-volatile component in smoke with headspace and solid phase microextraction was proposed.Based on the principle of the greatest amount of information content,carboxen/ polydimeylsiloxane fiber was applied in solid phase microextraction with extraction temperature of 70 ℃ and extraction time of 60 min.Agilent DB-1 chromatographic column was used for GC separation and the full-scanning mode was adopted in MS determination.It was found that 107 components were identified.The highest content was nicotine (18.07%),and the higher content was neophytadiene (11.46%).The main volatile and semi-volatile components were benzene series,ketones,and heterocyclic compounds.