荷移分光光度法测定氨酪酸
Spectrophotometric Determination of Aminobutyric Acid by Charge Transfer Reaction
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摘要: 研究了氨酪酸(ABA)与2,3-二氯-5,6-二氰-1,4-苯醌的荷移反应.确定在硼砂缓冲溶液中,于50 ℃水浴中恒温40 min,可获得1∶1的络合物,其λmax=340 nm,表观摩尔吸光率ε=1.37×103L·moL-1·cm-1.用拟定方法测定片剂中氨酪酸的含量与标准方法一致,回收率为98.5%~101.9%,相对标准偏差为1.3%~1.9%.Abstract: In a borate buffer medium of pH 9.3,a charge transfer complex with mole ratio of 1 to 1 was formed between aminobutyric acid (ABA) and 2,3-dichloro-5,6-dicyano-benzoquinone (DDBQ) heated at 50 ℃ for 40 min in a water-bath,with its absorption maxima at 340 nm and its molar absorptivity (ε340) of 1.37×103L·mol-1·cm-1.The proposed method has been applied to the analysis of drug tablets for its content of aminobutyric acid,giving RSD′s in the range 1.3% to 1.9%,and recoveries in the range of 98.5%-101.9%.By checking the results obtained by this method with the results obtained by the method of national standard,no significant deviation was observed as proved by the t-test.