牛血红蛋白催化分光光度法测定过氧化氢
Spectrophotometric Determination of Hydrogen Peroxide with Bovine Hemoglobin as Catalyst
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摘要: 在pH 4.8的0.1 mol·L-1柠檬酸-0.2 mol·L-1磷酸氢二钠缓冲介质中,于43 ℃水浴加热60 min,牛血红蛋白对过氧化氢氧化邻苯二胺的反应有明显的催化作用。试验结果表明: 过氧化氢浓度在8.24×10-6~2.31×10-4mol·L-1范围内与相应吸光度的增加值ΔA呈线性关系,检出限(3s/k)为4.50×10-8mol·L-1。据此提出了牛血红蛋白催化分光光度法测定过氧化氢的含量。采用此法测定消毒液和雨水中过氧化氢的含量,测定值的相对标准偏差(n=5)分别为0.081%和1.6%, 消毒液中过氧化氢的测定值与滴定法测定结果相符。以上述2种试样作基体用标准加入法进行回收试验,测得回收率分别为99.3%和97.3%。Abstract: In a buffer mediun of 0.1 mol·L-1 citric acid solution mixed with 0.2 mol·L-1 Na2HPO4 solution of pH 4.8 and heated in a water bath at 43 ℃ for 60min, the oxidation of o-phenylenediamine by hydrogen peroxide was remarkably catalyzed by the present of bovine hemoglobin. Linear relationship between values of increase in absorbance (ΔA) and concentration of hydrogen peroxide was obtained in the range of 8.24×10-6-2.31×10-4mol·L-1 with detection limit (3s/k) of 4.50×10-8mol·L-1 . Based on these facts, a spectrophotometric method for determination of hydrogen peroxide with bovine hemoglobin as catalyst was proposed. The proposed method was applied to the determination of hydrogen peroxide in samples of disinfectant liquor and rainwater, RSD′s (n=5) calculated from the determined values were 0.081% and 1.6% respectively. The determined value of hydrogen peroxide in the disinfactant liquor was found to check quite well with the result found by titrimetry. Test for recovery was performed by standard addition method using these 2 samples as matrixes, values of recovery found were 99.3% and 97.3% respectively.
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