Abstract: HPLC fingerprint atlas of flavonoides in the TCM of leaves of Microcos paniculata L. was established by using Kromasil C18 column (250 mm×4.6 mm, 5 μm) as stationary phase and mixed solutions of methanol and dil. phosphoric acid solution (0.1+99.9) in different ratio as mobile phase for gradient elution in the HPLC analysis. Vitexin was used as internal standard and UV-detection at the wavelength of 276 nm was adopted. Samples of the TCM were taken from 9 producing areas in our country and analyzed by the proposed method. HPLC fingerprint atlas showing 18 peaks of flavonoides of the 9 TCM′s was obtained, with good similarities among data of relative retention time and relative peak area of the 9 samples. The established fingerprint was proved to be feasible for use in the quality control of the TCM.