一种多卤代苯基荧光酮显色剂的合成及分光光度法测定微量镉

Synthesis of a Multi-Halogenated Phenylfluorone Chromogenic Reagent and Spectrophotometric Determination of Trace Amount of Cadmium

摘要: 合成了一种新型的多卤代苯基荧光酮显色剂2′,4′-二氯苯基-4,5-二溴荧光酮(DCDBPF)。在pH 10的十六烷基三甲基溴化胺-辛烷基酚聚氧乙烯醚微乳液中,镉(Ⅱ)能与DCDBPF发生显色反应形成1比3的稳定配合物,配合物的最大吸收波长位于626 nm,其摩尔吸光率为1.0×105mol-1·L·cm-1。镉的质量浓度在1.8 mg·L-1以内符合朗伯-比耳定律,检出限为10 μg·L-1。方法用于墨鱼样品的分析,加标回收率在95.2%~97.3%之间,测定值的相对标准偏差在2.5%~4.8%之间。

Abstract: A novel multi-halogenated phenylfluorone chromogenic reagent, 2′,4′-dichlorophenyl-4,5-dibromofluorone (DCDBPF) was synthesized. Cd(Ⅱ) could react with DCDBPF to form an stable color complex at ratio of 1:3 in pH 10 solution containing CTMAB-OP microemulsion. The λmax of the color complex found was 626nm and the apparent molar absorption coefficient found was 1.0×105mol-1·L·cm-1. Mass concentration of Cd(Ⅱ) followed the Beer′s law within 1.8 mg·L-1, with detection limit of 10 μ g·L-1. The proposed method was applied to the analysis of cuttlefish samples, giving values of recovery and RSD′s in the ranges of 95.2%-97.3% and 2.5%-4.8%, respectively.