蒸发光散射检测-高效液相色谱法测定沙棘果蜜中的葡萄糖、果糖和蔗糖

Determination of Glucose, Fructoes and Sucrose in Seabuckthorn Fruit Honey by HPLC with ELSD

摘要: 提出了高效液相色谱法测定沙棘果蜜中葡萄糖、果糖和蔗糖含量的方法。以Zorbax-NH2为色谱柱(4.6 mm×250 mm,5 μm),乙腈-水(90+10)混合溶液为流动相进行淋洗分离,用蒸发光散射检测器检测。峰面积的对数值与3种糖的质量浓度的对数值在10~1 000 mg·L-1范围内呈线性关系,方法的检出限(3S/N)均为10 mg·L-1。采用标准加入法做回收试验,平均回收率在95.0%~105.0%之间,相对标准偏差(n=5)均小于2.5%。

Abstract: HPLC was applied to the determinations of glucose, fructoes and sucrose in seabuckthorn fruit honey. The 3 saccharides were separated on Zorbax-NH2 (4.6 mm×250 mm, 5 μm) chromatographic column with a mixture of acetonitrile and water (90+10) as mobile phase, and their contents determined by evaporation light scattering detector (ELSD). Linear relationship between logarithmic values of peak area and mass concentration of the 3 saccharides were obtained in the same range of 10-1 000 mg·L-1 and with same detection limit (3S/N) of 10 mg·L-1. The values of average recovery found by standard addition method were in the range of 95.0%-105.0%, with values of RSD′s (n=5) less than 2.5%.