液相色谱-串联质联法测定食品用塑料包装中邻苯二甲酸酯类增塑剂
LC-MS/MS Determination of Phthalates in Plastic Food Packaging
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摘要: 采用液相色谱-串联质谱法(LC-MS/MS)测定了食品用塑料包装材料中的3种邻苯二甲酸酯(DBP、BBP、DEHP)的含量。样品经正己烷超声提取后,以Hypersil Gold色谱柱为固定相,以甲醇-水(95+5)溶液为流动相进行洗脱,采用电喷雾正离子源-多反应检测模式检测。3种邻苯二甲酸酯的质量浓度在5.00~250 μg·L-1范围内与其峰面积呈线性关系,方法的检出限(3S/N)在0.1~0.2 μg·L-1之间。对空白样品进行加标回收试验,回收率在92.5%~101%之间,测定值的相对标准偏差(n=6)在4.6%~6.9%之间。Abstract: LC-MS/MS was applied to determine three phthalates (DBP, BBP and DEHP) in plastic food packaging. The sample was extracted with hexane by ultrasonic. Hypersil Gold column was used as stationary phase and the mixture of methane and water was used as mobile phase. ESI+ and MRM were adopted in MS/MS. Linear relationship between the peak area and the mass concentration of phathalates in the range of 5.00-250 μg·L-1, with detection limits (3S/N) in the range of 0.1-0.2 μg·L-1. Recovery measured by standard addition method was in the range of 92.5%-101% with RSD′s (n=6) in the range of 4.6%-6.9%.
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