顶空萃取-气相色谱-质谱法分析马鞭草的挥发油组分

Head-space Extraction-GC/MS Determination of Chemical Components of Volatile Oil from Verbena

摘要: 采用顶空萃取-气相色谱-质谱法分离和鉴定马鞭草挥发油化学成分,用归一化法测定其相对含量.鉴定出64个组分,其含量占总挥发油组分峰面积的97.76%.主要成分是乙酸(3.55%)、芳樟醇(4.41%)、反-石竹烯(9.30%)、反-β-金合欢烯(3.99%)、律草烯(5.61%)、α-姜黄烯(8.50%)、十五烷(8.48%)、γ-芹子烯(3.75%)、β-没药烯(5.66%)、β-杜松烯(3.57%).

Abstract: Conditions for head-space extraction were given,and the volatile oil in the sample was introduced into the hyphenated GC/MS instrument for separation and identification of its chemical components.The relative contents(in %) of the components were determined using the method of normalization.Seventy four components were found in the separation,among which 64 components were identified.The sum of peak areas of the identified components was found to account for 97.76% of the total peak areas of components in volatile oil.The main compounds among the 64 components are the following: acetic acid(3.55%),linalool(4.41%),α-humulene(5.61%),trans-caryophyllen(9.30%),trans-β-farnesene(3.99%),α-curcumene(8.50%),pentadecane(8.48%),γ-selinene(3.75%),β-bisabolene(5.66%) and β-cadinene(3.57%).