高效液相色谱法测定双黄连制剂中绿原酸和黄芩苷

HPLC Simultaneous Determination of Chlorogenic Acid and Baicalin in Shuanghuanglian Preparations

摘要: 建立同时测定双黄连制剂中绿原酸(CGA)和黄芩苷(BCL)的高效液相色谱法.采用梯度洗脱,色谱柱为Waters Symmetry C18(3.9 mm×150 mm,5 μｍ)柱,以甲醇-0.06 mol·L-1磷酸为流动相,流速为0.8 mL·min-1,检测波长为320 nm.绿原酸的线性范围为1.72~137.6 mg·L-1,回归方程y=-28 900.12+23 036.71 x(r=0.999 6),平均回收率为95.8%,相对标准偏差为3.53%(n=7);黄芩苷的线性范围为3.35~267.9 mg·L-1,回归方程y=19 940.40+13 564.15 x(r=0.999 8),平均回收率为97.3%,相对标准偏差为2.85%(n=7).

Abstract: HPLC was applied to the simultaneous determination of chlorogenic acid and baicalin in “Shuanghuanglian” compound preparations.Waters Symmetry C18 column (3.9 mm×150 mm,5 μm) was used in the chromatographic separation,and a solution containing methanol and dilute.phosphoric acid was used as the mobile phase in the gradient elution,with a flow-rate of 0.8 mL·min-1.UV-detection at 320 nm was selected in the determination.Linearity range for determination of chlorogenic acid was found in the range of 1.72-137.6 mg·L-1 (y=28 900.12+23 036.71 x,r=0.999 6),and for determination of baicalin in the range of 3.35-267.9 mg·L-1 (y=19 940.40+13 564.15 x,r=0.999 8).The RSD′s found in the precision test were 3.53% (n=7) for chlorogenic acid,and 2.85% (n=7) for baicalin,and average recovery obtained were 95.8% and 97.3% respectively.