β-环糊精修饰区带毛细管电泳法快速测定染料木甙、染料木素、山柰酚和芦丁

RAPID DETERMINATION OF GENISTIN,GENISTEIN,KAEMPFEROL AND RUTIN BY CAPILLARY ZONE ELECTROPHORESIS MODIFIED BY β-CYCLODEXTRIN

摘要: 通过研究缓冲溶液的pH和浓度、β-环糊精浓度、十二烷基硫酸钠浓度和毛细管温度等条件,建立了一种β-环糊精修饰区带毛细管电泳法快速测定染料木甙、染料木素、山柰酚和芦丁的方法.在优化的条件下,4种物质在6 min内得到良好分离.染料木甙、染料木素、山柰酚和芦丁峰面积和质量浓度分别在2-400,2-300,2-200和5-500 mg·L-1范围内呈线性关系.染料木甙、染料木素、山柰酚和芦丁的检出限分别为0.8,0.6,0.6和1.4 mg·L-1.方法用于分析生槐角和炙槐角样品并做加标回收试验,回收率结果在93.8%-105.2%.

Abstract: Through a thorough study of the working conditions,including the concentration and pH of buffer solution,concentration of β-CD and SDS in the solution and the temperature of capillary,etc.,a rapid method of β-CD modified capillary zone electrophoretic determination of genistin,genistein,koenpferol and rutin was proposed.Under the optimum condition,4 compounds mentioned above were separated nicely within 6 min.Linear relationship between the peak area and concentration of these 4 compounds were obtained in the ranges of 2-400,2-300,2-200 and 5-500 mg·L-1 for genistin,genistein,kaempferol and rutin with detection limits of 0.8,0.6,0.6 and 1.4 mg·L-1 respectively.The proposed method has been applied to the analysis of fructus sophorae samples,giving recoveries in the range of 93.8%-105.2%.