Study of Extraction and Antioxidation of Polysaccharides in Chinese Honeylocust Spine

Extraction and antioxidation of polysaccharides in Chinese honeylocust spine was studied.The polysaccharide content of Chinese honeylocust spine was extracted with ultrasonic assisted technology and determined by phenol-sulfuric acid method using glucose as standard reference at the wavelength of 484 nm.The optimized conditions found by a series single-factor test and L9(34) orthogonal experiment were as follows:extraction temperature was 50 ℃;pH was 7;extraction time was 40 min and ratio of liquid to solid was 30∶1.Extraction yield of polysaccharide in Chinese honeylocust spine found was 1.26%.The antioxidative experiments indicated that removal rates of ·OH,DPPH· and ·O2- found were 88.9%,89.3% and 42.3%,respectively at mass concentration of polysaccharide in Chinese honeylocust spine of 2.5 g·L-1.