HPLC-MS/MS Simultaneous Determination of Residual Amounts of Nitrofurans and Nitroimidazoles in Aquatic Products

HPLC-MS/MS was applied to the simultaneous determination of residual amounts of nitrofurans and nitroimidazoles in aquatic products.The sample was hydrolyzed in hydrochloric acid medium,and derivatized in an acidic condition for 16 h.Ethyl acetate was used as extraction solvent.Phenomenex Luna C18 chromatographic column was used as stationary phase,and mixtures of acetonitrile and acetic acid solution (0.2+99.8) mixed in different ratio were used as mobile phase in gradient elution.ESI+ and multi-reaction monitoring were adopted in MS/MS analysis.Isotope was used as internal standard.Detection limits (3S/N) found were ranged from 0.15 to 0.30 μg·kg-1.On the base of blank sample,test for recovery was made by standard addition method,values of recovery found were in the range of 83.3%-115%,with RSD′s (n=6) in the range of 2.3%-14%.