Determination of Rg1, Re and Rb1 in Intermediate Products of ShenMai Injection by UHPLC

UHPLC was applied to the determination of ginsenosides (i.e., Rg1, Re and Rb1) in the intermediate products of ShenMai injections. The sample of intermediates was filtrated through 0.2 μm membrane filter. The filtrate was separated on ACQUITY UPLC shield BEH RP18 column with a mixture of acetonitrile and water as mobile phase in gradient elution. UV detection was made at the wavelength of 203 nm. Linear relationships between values of peak areas and mass concentrations of the Ginsenosides were kept in the same range of 0.081 8-0.409 2 g·L-1, with detection limits (3S/N) of 0.652, 0.479, 0.916 mg·L-1. Values of recovery found by standard addition method were in the range of 99.9%-100% , with RSD′s (n=6) in the range of 0.47%-0.64%.