Determination of Aziridine by HPLC

A HPLC was applied to the determination of aziridine. Self-made aziridine monomer was derivatized with Folin′s reagent (1,2-naphthoquione-4-sulphonic acid) and the structure of the derivate was characterized by NMR and IR. For determination of aziridine, the derivate was seperated on Agilent ODB-C18 (250 mm×4.6 mm, 5 μm) column, using a mixtrure of acetonitrile and 0.04 mol·L-1 sodium phosphate dibasic dodecahydrate solution as mobile phase in gradient elution. The wavelength used in UV-detection was 258 nm. The linearity range for aziridine was in the range of 1.00-20.0 mg·L-1, the detection limit (3S/N) was 0.05 mg·L-1, and RSD′s (n=6) found were less than 2%.