HPLC Determination of Residual Amounts of Clenbuterol in Pork and Serum

HPLC was applied to the determination of residual amounts of clenbuterol in pork and serum. The sample was extracted with a mixture of methanol and 0.01 mol·L-1 hydrochloric acid (68+32) and after purification with ethylenediamine-N-propylsilane, the sample solution was separated on Zorbax Eclipse C18 chromatographic column with a mixture of methanol and sodium dihydrogen phosphate solution (68+32) as mobile phase. UV detection was made at the wavelength of 270 nm. Linearity range of clenbuterol was between 0.01-5.00 mg·L-1, with detection limit (3S/N) of 1.8 μg·kg-1. Values of recovery obtained by standard addition method were in the range of 87.4%-99.2% , with RSDs (n=5) in the range of 2.1%-2.8%.