Determination of Hydrogen Peroxide in Hair Dye by Fluorescence Spectrometry Base on Hemin Catalysis

A method for the determination of hydrogen peroxide in hair dye by fluorescence spectrometry was developed based on that, the p-hydroxyphenylacetic acid could be oxidized by hydrogen peroxide with hemin as mimetic catalyzer to form the dimer with strong fluorescence. The optimum conditions for maximum fluorescence intensity were as follow: 10 μmol·L-1 of hemin, 1 mmol·L-1 of p-hydroxyphenylacetic acid, pH 10, reaction at room temperature for 5 min, λex of 310 nm and λem of 400 nm. Linear relationship was found between the fluorescence intensity and the concentration of hydrogen peroxide in the range of 0.05-50 μmol·L-1, with detection limit (3S/N) of 0.3 μg·kg-1. Recovery rates obtained by standard addition method were in the range of 94.5%-105% with RSDs (n=6) less than 2%.