UHPLC-MS/MS Determination of Abietic Acid and 11-Carbonyl-β- acetyl-boswellic Acid in Gentongping Capsules and Tablets

The sample (0.200 0 g) was extracted ultrasonically with ethanol for 30 min, and diluted to 10.0 mL with ethanol. The extract was separated by UHPLC using Zorbax SB-C₁₈ column as stationary phase, and mixture of acetonitrile and 0.1% (φ) formic acid solution (17+3) as mobile phase. In MS analysis, ESI⁺ and MRM were adopted. Quantification was attained by external standard method with preparation of standard curves by matrix-matching method. As shown by the results, linear relationships between values of peak area and mass concentration of abietic acid and 11-carbonyl-β-acetyl boswellic acid were found in the same range of 10.0-1 000 μg·L^-1, and the detection limits (3S/N) found were 0.9, 3.1 μg·kg^-1 respectively. Test for recovery was made by standard addition method at 3 concentration levels, giving results in the range of 95.2%-101%. Values of the inter-and intra-day precision (RSDs, n=6) were all less than 9%.