UHPLC Determination of 3 Isothiazolinone Bactericides in Water-Based Adhesive for Cigarettes

UHPLC combined with ultrasonic extraction was applied to the simultaneous determination of 3 isothiazolinones in water-based adhesive for cigarettes. The sample (0.300 0 g) was extracted by ultrasonic with 20 mL of methanol solution (1+1) for 30 min. The extract was separated on a Waters ACQUITY UPLC HSS T3 chromatographic column with the mixture of water and acetonitrile as mobile phase in gradient elution. Anlytes were detected by diode array detector at the wavelength of 275 nm and 318 nm. Linear relationships were found between values of peak area and mass concentration of the 3 isothiazolinones in definite ranges, with detection limits (3S/N) in the range of 0.005-0.01 mg·L^-1, and lower limits of determination (10S/N) in the range of 0.015-0.03 mg·L^-1. Recoveries obtained by standard addition method were in the range of 92.2%-100% and RSDs (n=6) were in the range of 1.2%-6.0%.