UHPLC-MS/MS Determination of 10 Weight Loss Compounds in Slimming Health Foods

The sample (0.500 0 g) was extracted ultrasonically for 20 min with 25 mL of methanol, and the extract was diluted to 50 mL with methanol, and filtered through microporous membrane of 0.22 μm. The solution was then separated by UHPLC using Thermo Hypersil Gold column as stationary phase and a mixture of acetonitrile and 0.1% (φ) formic acid solution in various ratios as mobile phase in gradient elution. ESI with switching mode of positive ions and negative ions and MRM were adopted in MS. Linear relationships between values of peak area and mass concentration of 10 compounds were found in definite ranges, with detection limits (3S/N) in the range of 0.01-1.00 μg·g^-1. Test for recovery was made by standard addition method, giving results in the range of 72.1%-117%, with RSDs (n=5) ranged from 0.70% to 6.2%.