UV-Spectrophotometric Determination of Rutin in Medicine and Food Products with Separation by SPE Using Ionic Liquid Loaded β-Cyclodextrin-Cross-Linked Polymer as Solid Extractant

Self-prepared cross-linked polymer formed between imidazole ionic liquid and β-cyclodextrin (IL-β-CDCP) was used as solid extractant in the SPE of rutin from samples of medicine and food, and optimum conditions for the separation were studied. The analyte (rutin) was then determined by UV-spectrophotometry. As shown by the results, when 0.1 g of IL-β-CDCP was added to 40 mL of acetate buffer solution (pH 4.0) containing rutin, and the mixture was shaked for 10.0 min in a water bath heated at 15.0℃, 92.0% of the rutin was found adsorbed on IL-β-CDCP. After centrifugation, and removing the super liquid phase, absolute alcohol (4.0 mL) was added to the IL-β-CDCP adsorbed with rutin settled in the lower phase, and the mixture was shaked for 10.0 min at 35.0℃ in a water bath, during which 94.0% of the adsorbed rutin was eluted from IL-β-CDCP and dissolved in alcohol. The absorbance of the alcohol solution was measured at 360 nm. Linear relationship between values of absorbance and mass concentration of rutin was found in the range of 0.06 to 7.0 mg·L^-1, with detection limit (3s/k) of 8.1 μg·L^-1. Values of RSD's (n=6) found were range from 0.38% to 1.7%.